Subategory:

Mutants

Description:

Genotype: mt-; arg7-8; nuo5::APHVII; NUO5K230R-FLAG; APHVIII. This is the amc9 strain complemented with the NUO5 gene containing the K230R substitution with a C-terminal FLAG tag. It was created by mutagenesis of amc9 with plasmid pRS426 containing the NUO5 gene encoding the K230R substitution with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant. It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine.

Species:

Chlamydomonas