Subategory:

Mutants

Description:

This strain expresses IFT81(2E) in a background null for IFT81. IFT81(2E) is a version of IFT81 in which two highly conserved basic residues (K73 and R75) in the protein's calponin-homology domain, implicated in binding to tubulin, have been replaced by glutamate. The strain has apparently normal length flagella, normal flagellar regeneration kinetics, normal motility, and normal IFT. The frequency of anterograde tubulin IFT was not significantly reduced in the flagella of this strain. It was created by insertional mutagenesis of the parent strain with a fragment conferring resistance to hygromycin B (aph7") to generate ift81-1, followed by transformation with a DNA fragment encoding IFT81(2E) and containing a paromomycin-resistance gene as a selectable marker. Mutant allele: ift81-1, chromosome_17:3362408-3368081.

Species:

Chlamydomonas

Locus:

IFT81

Chromosome:

17