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MUT-5963 ChR2-E90R, ΔChR1 (E162) [PH226]
Datasheet
Catalog No: ALS-04886
Product Details
Subategory:
Mutants
Description:
This is a ChR1 disruption strain with a point mutation E90R in ChR2, generated with SpCas9 nuclease. Background strain: MUT-125 mt+. Nuclease: SpCas9 nuclease. Target gene: ChR1, Cre14.g611300; ChR2, Cre02.g085257. Target sequence: ChR1 TGTGGCTTCGTTACGCGGAG (exon 5); ChR2 CTATGTGTGCGCTATCGAGG (exon 4). Marker: pAphVII (pPH360), pAphVIII (pPH114). Mutation: E90R exchange in ChR2; insertion of short oligo TTTCGATTGAAGGAAAAGTTACAACGGAGT in ChR1.
Species:
Chlamydomonas
Locus:
ChR1, Cre14.g611300; ChR2, Cre02.g085257
Chromosome:
14, 2
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For Research Use Only. Not For Clinical Use.