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MUT-5966 PH180-cross-CC124 [PH215]
Datasheet
Catalog No: ALS-04889
Product Details
Subategory:
Mutants
Description:
This is a ChR2 disruption strain with a point mutation E162T in ChR1, generated with SpCas9 nuclease in MUT-125 and crossed into MUT-124. Background strain: MUT-124 mt-. Nuclease: SpCas9 nuclease. Target gene: ChR1, Cre14.g611300; ChR2, Cre02.g085257. Target sequence: ChR1 TGTGGCTTCGTTACGCGGAG (exon 5); ChR2 CTATGTGTGCGCTATCGAGG (exon 4). Mutation: E162T exchange in ChR1; insertion of short oligo TTAGCTAAGCCTCCCCAAAGCCTGGCCAGGGTCTAG in ChR2.
Species:
Chlamydomonas
Locus:
ChR1, Cre14.g611300; ChR2, Cre02.g085257
Chromosome:
14, 2
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For Research Use Only. Not For Clinical Use.