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MUT-5969 ΔSNRK2.2-C10 [PH159]
Catalog No: ALS-04892
Product Details
Subategory:
Mutants
Description:
This is a SNRK2.2 (SAC3) disruption strain, generated with CRISPR/Cas9. Clone C10-A10. Mutants with a disrupted SNRK2.2 (SAC3) gene show constitutive arylsulfatase expression and can phenotypically screened with X-SO4 dyes. Background strain: SAG11-32b (=MUT-409). Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP). Marker: pAPHVIII (pPH75). Target gene: SNRK2.2, Cre12.g499500. Target sequence: TAGCGAGGATGTCCAATCAG GGG (exon 1). Mutation: insertion of short oligo (TTAGACTCTAACTAGATCAGcgg).
Species:
Chlamydomonas
Locus:
SNRK2.2, Cre12.g499500
Chromosome:
12
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For Research Use Only. Not For Clinical Use.