Subategory:

Mutants

Description:

Strains carrying the ac46 mutation have a strong tendency to acquire second-site suppressors, but we have always been able to recover the original mutation by outcrossing to wild type. The y1 mutant is a better genetic marker for this linkage group. MUT-705 is from MUT-125 x MUT-563, a suppressed ac46 strain no longer in the collection. It was scored as cleanly acetate-requiring. In a cross of MUT-705 x MUT-124, complete tetrads were obtained with clear 2:2 segregation. MUT-705 was subsequently outcrossed to produce MUT-1361. The ac46 mutant appears to map within 1-2 map units of its own centromere but in early crosses did not show linkage to any of 36 markers tested on other linkage groups. Crosses showed that AC46 and Y1 constitute a single linkage group. It is demonstrated that the ac46 locus affects expression of the chloroplast atpH and atpI genes encoding subunits of chloroplast ATP synthase.

Species:

Chlamydomonas

Locus:

AC46

Chromosome:

15

Phenotype:

Requires acetate (suppressed)