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P-183 cpDNA BamHI 11/12 (7.0 kb) sr spr er
Datasheet
Catalog No: ALRK-03067
Product Details
Description:
Chloroplast DNA BamHI 11/12 (7.0 kb) in pUC18; 16S through 5' end of 23S rRNA gene; contains sr-u-2-60, spr-u-1-6-2, and er-u-37 mutations. Note: by selecting cells transformed with this plasmid simultaneously for spectinomycin and streptomycin or erythromycin resistance one essentially eliminates the possibility of recovering spontaneous antibiotic resistant mutants. However, cells carrying both spr-u-1-6-2 and sr-u-2-60 have been found to be deficient in Chloroplast protein synthesis and have impaired photosynthetic performance under high iradiance conditions, consistent with reduced levels of D1 synthesis. Thus if selection for antibiotic resistance is to be used specifically for cotransformation, we recommend using P-228 (spr-u-1-6-2 alone) instead.
Strain:
MUT-227 spr-u-1-6-2 sr-u-2-60 er-u-37
Insert:
BamHI 11/12 (7.0 kb)
Genes Present:
16s rRNA, 23s rRNA (5' end)
Vector:
pUC18
Bacterial Host Strain:
JM83
Selectable Marker:
Amp-r, white on X-gal
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For Research Use Only. Not For Clinical Use.