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P-228 cpDNA BamHI-HindIII (4.8 kb) spr-u-1-6-2
Catalog No: ALRK-03081
Product Details
Description:
Chloroplast DNA Bam-HindIII (4.8 kb) in pUC18; 16S through 5' part of 23S rRNA gene; from MUT-110, contains spectinomycin resistance mutation spr-u-1-6-2; see P-667 for the same insert in an XL1 blue strain. The spr-u-1-6-2 mutations is an A-> G change at bp 1123 in the C. reinhardtii 16S rRNA gene which causes loss of an AatII restriction site and confers high level spectinomycin resistance. Used in rRNA transformation experiments.
Strain:
MUT-110 spr-u-1-6-2 mt+
Insert:
BamHI-HindIII (4.8 kb) from BamHI 11/12
Genes Present:
16s rRNA, 23s rRNA (5' end)
Vector:
pUC18
Bacterial Host Strain:
JM83
Selectable Marker:
Amp-r, white on X-gal
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For Research Use Only. Not For Clinical Use.