Description:

Chloroplast DNA BamHI-HindIII (5.9 kb) in pUC18, from C. smithii; contains 16S through 5' end of 23S, with spectinomycin resistance mutation near 3' end of 16S rRNA. The DNA in this strain came from MUT-2477, which was a spectinomycin-resistant isolate from the C. smithii/C. reinhardtii hybrid strain MUT-1852. The Chloroplast genome in this strain was derived from C. smithii. MUT-2477 is a spectinomycin-resistant derivative of MUT-1852, a hybrid between C. smithii (MUT-1373) and C. reinhardtii (MUT-124). The MUT-124 parent was treated with acriflavine to eliminate mitochondrial DNA before mating, so MUT-1852 and its derivatives contain the C. smithii versions of both Chloroplast and mitochondrial DNA in a hybrid nuclear background. The spectinomycin resistance mutation is an A-> G change at position 1123 in the C. reinhardtii 16S rRNA gene that confers high level spectinomycin resistance and destroys an AatII restriction site.

Strain:

MUT-2477 C. smithii / C. reinhardtii hybrid

Insert:

BamHI-HindIII (5.9 kb)

Genes Present:

16s rRNA, 23s rRNA (5' end)

Vector:

pUC18

Bacterial Host Strain:

JM83

Selectable Marker:

Amp-r, white on X-gal