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P-230 cpDNA AatII (0.28 kb) SDR probe
Datasheet
Catalog No: ALRK-03083
Product Details
Description:
Chloroplast DNA 0.28 kb AatII fragment in pUC18, from BamHI 11/12, MUT-125 wild type; spacer region between 16S and 23S rRNA genes; used as a probe for short dispersed repeat sequences in the Chloroplast genome. The insert is subclone of Bam 11/12, located within the spacer region between the 16S and 23S rRNA genes and containing short dispersed repeat elements. The AatII fragment was treated with Klenow to generate blunt ends and cloned into the SmaI site of pUC18. The AatII and SmaI sites have been lost but the insert can be excised using restriction sites in the pUC18 polylinker.
Strain:
MUT-125 wild type mt+
Insert:
AatII (0.28 kb)
Vector:
pUC18
Bacterial Host Strain:
JM83
Selectable Marker:
Amp-r, white on X-gal
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For Research Use Only. Not For Clinical Use.