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P-240 cpDNA KpnI (3.0 kb) C. smithii spr
Datasheet
Catalog No: ALRK-03087
Product Details
Description:
Chloroplast DNA KpnI (3.0 kb) in pUC18; 16S rRNA gene from C. smithii spectinomycin resistant mutant. The DNA in this strain came from MUT-2477, which was a spectinomycin-resistant isolate from the C. smithii/C. reinhardtii hybrid strain MUT-1852. The Chloroplast genome in this strain was derived from C. smithii. MUT-2477 is a spectinomycin-resistant derivative of MUT-1852, a hybrid between C. smithii (MUT-1373) and C. reinhardtii (MUT-124). The MUT-124 parent was treated with acriflavine to eliminate mitochondrial DNA before mating, so MUT-1852 and its derivatives contain the C. smithii versions of both Chloroplast and mitochondrial DNA in a hybrid nuclear background.
Strain:
MUT-2477 C. smithii / C. reinhardtii hybrid
Insert:
KpnI (3.0 kb)
Genes Present:
16s rRNA
Vector:
pUC18
Bacterial Host Strain:
JM83
Selectable Marker:
Amp-r, white on X-gal
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For Research Use Only. Not For Clinical Use.