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P-259 cpDNA XhoI 8 (11.0 kb) er-u-1a
Datasheet
Catalog No: ALRK-03089
Product Details
Description:
Chloroplast DNA XhoI 8 (11.0 kb) in BSKS+; contains psbA, 5S, and 3' end of 23S with er-u-1a mutation from MUT-64. This map is from the original file for the plasmid and was drawn before the complete sequence was known. The er-u-1a mutation is a C->T change at bp 2622 in C. reinhardtii 23S rRNA gene (equivalent to E. coli 2611), which confers erythromycin resistance in Chlamydomonas. The insert was cloned in the XhoI site of the BSKS+ polylinker in both orientations. In P-259A, the psbA gene direction is from the KS SacI site towards the KS KpnI site; in P-259B, the psbA direction is from the KS KpnI site towards the KS SacI site.
Strain:
MUT-64 er-u-1a mt+
Insert:
XhoI 8 (11.0 kb)
Genes Present:
psbA, 5s rRNA, 23s rRNA (small exon)
Vector:
BSKS+
Bacterial Host Strain:
JM109
Selectable Marker:
Amp-r, white on X-gal
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For Research Use Only. Not For Clinical Use.