Products
Online Inquiry
P-261 cpDNA EcoRI (5.51 kb) er-u-1a
Datasheet
Catalog No: ALRK-03091
Product Details
Description:
Chloroplast DNA EcoRI (5.51 kb) in pUC8 contains 3' end of psbA, 5S, and 3' exon of 23S with er-u-1a mutation from MUT-64. The er-u-1a mutation is a C->T change at bp 2622 in the C. reinhardtii 23S rRNA gene (equivalent to E. coli 2611), which confers erythromycin resistance in Chlamydomonas. This is a subclone of P-259B. The insert contains sequences from the EcoRI site internal to psbA up to the EcoRI site in KS+, thus including some sequences of the SK+ polylinker beyond the original XhoI border of the insert in P-259B. Orientation of psbA is from the pUC8 Eco site toward the pUC8 HindIII site.
Strain:
MUT-64 er-u-1a mt+
Insert:
EcoRI (5.51 kb)
Genes Present:
psbA 3' end, 5s rRNA, 23s rRNA (small exon)
Vector:
pUC8
Bacterial Host Strain:
JM109
Selectable Marker:
Amp-r, white on X-gal
Related Products
For Research Use Only. Not For Clinical Use.