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P-262 cpDNA EcoRI (4.0 kb) er-u-1a
Datasheet
Catalog No: ALRK-03092
Product Details
Description:
Chloroplast DNA EcoRI (4.0 kb) in pUC8; contains 3' end of psbA, 5S, and 3' exon of 23S with er-u-1a mutation from MUT-64; 1.5 kb deletion in spacer between psbA and 5S. This is a subclone of P-261. The three KpnI fragments including the BamHI site (approx. 1.5 kb) located between the psbA and 5S genes were deleted, and the remaining two Kpn ends were religated, leaving only one KpnI site. The insert appears slightly smaller than 4 kb. The er-u-1a mutation is a C->T change at bp 2622 in C. reinhardtii 23S rRNA gene (equivalent to E. coli 2611), which confers erythromycin resistance in Chlamydomonas.
Strain:
MUT-64 er-u-1a mt+
Insert:
EcoRI (4.0 kb)
Genes Present:
psbA (exon 5), 5s rRNA, 23s rRNA (small exon)
Vector:
pUC8
Bacterial Host Strain:
JM109
Selectable Marker:
Amp-r, white on X-gal
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For Research Use Only. Not For Clinical Use.