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Description:

P-270 cpDNA BamHI-EcoRI (4.48 kb) rbcL disrupted

Catalog No: ALRK-03096

Product Details

Description:
Chloroplast DNA BamHI-EcoRI (4.48 kb) in pUC18 with Pst site deleted, rbcL gene disrupted with YEp24. The DNA was obtained from MUT-125 wild type. The insert contains the 0.48 kb Pst I fragment from yeast plasmid YEp24 (P-238), cloned into unique Pst site of a 4 kb Bam-Eco fragment of cpDNA, disrupting the rbcL gene. This construct was used in transformation experiments.
Strain:
MUT-125 wild type mt+
Insert:
BamHI-EcoRI (4.48 kb)
Genes Present:
rbcL, disrupted with 0.48 kb Pst I fragment from yeast plasmid YEp24
Vector:
pUC18 with PstI site deleted
Bacterial Host Strain:
JM83
Selectable Marker:
Amp-r

For Research Use Only. Not For Clinical Use.