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P-300 cpDNA Kpn I (3.1 kb) psbA R238K
Datasheet
Catalog No: ALRK-03106
Product Details
Description:
Chloroplast DNA Kpn I (3.1 kb) in BSKS+; psbA exons 4 and 5 with R238K mutation in exon 4. A 0.9 kb Eco RI fragment from P-260A (Eco sites located within 4th intron of psbA and BSKS polylinker) containing psbA exon 4 was mutated in vitro at position 238 arg -> lys and cloned in the EcoRI site of P-299 (2.3 kb KpnI-EcoRI fragment containing wild type sequences of psbA intron 4 and exon 5 cloned into BSKS+), yielding a 3.1 kb Kpn fragment inserted into BSKS+. This plasmid has the psbA Kpn 3.1 kb fragment with only the arg -> lys mutation.
Insert:
KpnI (3.1 kb)
Genes Present:
psbA (exons 4 and 5)
Vector:
BSKS+
Bacterial Host Strain:
JM109
Selectable Marker:
Amp-r, white on X-gal
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For Research Use Only. Not For Clinical Use.