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Description:

P-322 cpDNA EcoRI-XhoI (5.7 kb) DCMU4 er-u-1a

Inquiry Datasheet
Catalog No: ALRK-03109

Product Details

Description:
Chloroplast DNA EcoRI-XhoI (5.7 kb) in BSKS+; psbA exon 5 with DCMU4 mutation, 5S, and 23S 3' end with er-u-1a mutation. MUT-2534 = DCMU-4 er-u-1a. DCMU-4 mutation in 5th exon of C. reinhardtii psbA gene causes ser -> ala change at aa 264 D1 protein, conferring resistance to DCMU and atrazine; er-u-1a mutation is C ->T change at bp 2622 in C. reinhardtii 23S rRNA (equivalent to E. coli 2611), conferring resistance to erythromycin.
Strain:
MUT-2534
Insert:
Chloroplast DNA EcoRI-XhoI (5.7 kb)
Genes Present:
psbA (intron 4, exon 5); 5s rRNA, 23s rRNA (3' end)
Vector:
BSKS+
Bacterial Host Strain:
JM109
Selectable Marker:
Amp-r, white on X-gal

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For Research Use Only. Not For Clinical Use.