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P-361 cpDNA EcoRI-(XhoI) (3.6 kb) psbA DCMU4
Datasheet
Catalog No: ALRK-03111
Product Details
Description:
Chloroplast DNA EcoRI-(XhoI) (3.6 kb) in pUC18; psbA exon 5 with DCMU4 mutation, 5S and 23S 3' end; Kpn deletion between psbA and 5S. The DCMU-4 mutation in 5th exon of C. reinhardtii psbA gene causes a ser -> ala change at aa 264 of D1 protein, conferring resistance to DCMU and atrazine.
Strain:
MUT-2534 DCMU-4 er-u-1a
Insert:
2.2 kb Eco-Kpn fragment from P-322 containing 5th exon of psbA, ligated to 1.4 kb Kpn-Sal fragment from P-14 (Sal site located in BSKS polylinker) containing 5S rRNA gene and small exon of 23S rRNA gene. Resulting construct has Kpn fragments between psbA and 5S rRNA deleted as in P-320, but has wild type (erythromycin sensitive) 23S gene derived from P-14.
Genes Present:
psbA 23s rRNA, Kpn deletion
Vector:
pUC18
Bacterial Host Strain:
JM109
Selectable Marker:
Amp-r
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For Research Use Only. Not For Clinical Use.