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P-388 cpDNA KpnI-PstI (0.9 kb) psbA mutated
Datasheet
Catalog No: ALRK-03115
Product Details
Description:
Chloroplast DNA KpnI-PstI (0.9 kb) in KS+; psbA exon 5, mutated to stop codon at aa 345. The KS+ vector was digested with KpnI + PstI and a Kpn-Pst insert of 0.9 kb which includes exon 5 of psbA was inserted. This exon was mutated in vitro to change the aa codon ser-345 to a TAA stop codon followed by another change to create a new AflII restriction site. The oligo used in this mutagenesis was psbAstop. This construct was used to obtain the NP mutant.
Insert:
KpnI-PstI (0.9 kb)
Genes Present:
psbA exon 5
Vector:
KS+
Bacterial Host Strain:
JM109
Selectable Marker:
Amp-r
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For Research Use Only. Not For Clinical Use.