Description:

CRY1 marker; RPS14 gene, ca. 4 kb EcoRI fragment in pUC119. Derived by the following steps: pCRY1-1 was constructed by using PCR to amplify the 3.6 kb genomic region containing the entire CRY1-1 transcription unit and 250 bp of upstream sequence. The 3.6 kb product was cloned into pUC119 digested with XbaI and SacI. Plasmid pRS2-1 was constructed by amplifying a 1073 bp region of the RBCS2 promoter and cloning the PCR product into pUC119 deigested with SmaI and dT tailed with Taq polymerase. pJN1 was constructed by inserting the 3.0 kb BfrI-EcoRI fragment of pCRY1-1 into the HincII site of pRS2-1. pJN4 was constructed by ligating the 474 bp Alw26I-ApaI fragment of pCRY1-1 to the 7.2 kb ApaI-BamHI fragment of pJN1.