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P-667 cpDNA spectinomycin resistant spr-u-1-6-2
Datasheet
Catalog No: ALRK-03169
Product Details
Description:
Chloroplast DNA Bam-HindIII (4.8 kb) in pUC18, 16S rRNA through 5' part of 23S rRNA; contains spectinomycin resistance mutation spr-u-1-6-2, from MUT-110, in 3' end of 16S rRNA; same insert as P-228 but in XL1 blue host strain. This is the same insert as P-228, but in XL1 blue;The spr-u-1-6-2 mutations is an A-> G change at bp 1123 in the C. reinhardtii 16S rRNA gene which causes loss of an AatII restriction site and confers high level spectinomycin resistance.
Strain:
MUT-110 spr-u-1-6-2 mt+
Insert:
BamHI-HindIII (4.8 kb) from BamHI 11/12
Genes Present:
16s rRNA, 23s rRNA (5' end)
Vector:
pUC18
Bacterial Host Strain:
XL-1 blue
Selectable Marker:
Amp-r
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For Research Use Only. Not For Clinical Use.