Description:

Cloning vector for Staphylococcus aureus Cas9 (SaCas9) guide RNA transcription, controlled by the U6 snRNA promoter #4 (3' of Cre15.g640800). The 20 bp target site can be inserted in a cut-ligation reaction as annealed oligos into an Esp3I cloning site. The immediate 4-bp sequence upstream of the transcriptional start site was changed to ACTT in all U6 constructs to simplify the cloning procedures. Selection in C. reinhardtii: paromomycin. Selection in E. coli: ampicillin.

Vector:

Contains an aphVIII cassette for selection on paromomycin.

Bacterial Host Strain:

XL-1 blue