Description:

RSP3 genomic sequence as originally described as "pEK RS3". A 36 bp insertion was cloned into a Stu I site near the end of the RSP3 coding sequence, such that an HA epitope is encoded near the extreme carboxy-terminus of RSP3. Digestion with Eco R1 releases the entire genomic+tag insert (~4Kb) from the vector (3 Kb). Transformation of this plasmid into pf14 resulted in restoration of wild-type motility and the presence of HA-tagged RSP3 in flagella.

Selectable Marker:

Amp-r