Description:

pMJ504, containing the gDNA of Cre12.g498550, was reengineered by restriction digestion and T4 ligation to add in-frame immediately after the 3xFLAG a synthetic fragment containing three copies of the sequence coding for the 15 C-terminal amino acids of SAGA2 (Cre 09.g394621), interspersed with a short flexible linker (GGGGGS). Transformed into C. reinhardtii strain cMJ030 to produce MUT-5650. Selection in E. coli: ampicillin. Selection in C. reinhardtii: paromomycin.

Bacterial Host Strain:

DH5 alpha