Description:

Previously synthesized pMJ507 (CC-5652) was modified by side-directed mutagenesis. Triplet coding for Tryptophan in position 51 of the mature protein now codes for an Alanine. Triplet coding for Arginine in position 52 of the mature protein now codes for an Alanine. Transformed into C. reinhardtii strain cMJ030 to produce MUT-5653. Selection in E. coli: ampicillin. Selection in C. reinhardtii: paromomycin.

Bacterial Host Strain:

DH5 alpha