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pMJ509: pLM005-Cre10.g430350[W47A/R48A/W51A/R52A/W110A/R111A/W148A/R149A]-Venus-3xFLAG
Datasheet
Catalog No: ALRK-03325
Product Details
Description:
Previously synthesized pMJ507 (CC-5652) was modified by side-directed mutagenesis. All four triplet pairs coding for a Tryptophan-Arginine dipeptide were mutagenized to code for a double Alanine (W47A/R48A/W51A/R52A/W110A/R111A/W148A/R149A). Transformed into C. reinhardtii strain cMJ030 to produce MUT-5654. Selection in E. coli: ampicillin. Selection in C. reinhardtii: paromomycin.
Bacterial Host Strain:
DH5 alpha
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For Research Use Only. Not For Clinical Use.