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pOpt_cCA_mRuby2_Hyg
Datasheet
Catalog No: ALRK-03355
Product Details
Description:
Please follow naming convention for derivative vectors using underscores between new added elements. Individual annotated vector maps are available in ApE format.
Insert:
Optimized template vector containing the mRuby2 (RFP variant) reporter expression cassette (with N-terminal cCA secretion signal) and an antibiotic selection marker expression cassette. Both cassettes contain the HSP70A-RBCS2-InI promoter and RBCS2 3' UTR surrounding either the reporter or the marker. Each reporter contains intron 2 of RBCS2 within its nuclear sequence. Selection marker for Chlamydomonas is hygromycin B resistance aphVII.
Vector:
pBluescript backbone. Optimized synthetic Chlamydomonas expression cassettes.
Bacterial Host Strain:
DH5 alpha
Selectable Marker:
Amp-r
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For Research Use Only. Not For Clinical Use.