Description:

pWUCA2 can be used to introduce the trnWUCA gene downstream of psbH in the C. reinhardtii Chloroplast genome. trnWUCA is a synthetic tRNA that allows the readthrough of internal UGA codons. This plasmid also contains an empty expression cassette, where a gene of interest can be inserted (e.g. one with internal TGA codons). 100 μg/mL ampicillin in E. coli. Minimal medium for selection of restoration of psbH gene in C. reinhardtii (a psbH mutant recipient line must be used, e.g. TN72).