TALEN Service for Algae
Genome editing has a major role in advancing biotechnology and basic research. There is an urgent need for genetic editing of algae to improve the production of nutrients, proteins, and oils in algae.
As a company that has been providing gene editing services for many years, Lifeasible can provide high-quality and customizable gene editing services for algae based on TALEN (Transcription Activator-Like Effector Nuclease) technology.
Introduction to TALEN
TAL effectors in nature are imported into plant host cells by Xanthomonas campestris via a type III secretion system where they change the gene expression of the host to meet the needs of the bacteria. TALEN is an artificial chimeric protein, consists of a non-specific Fok I restriction endonuclease domain fused to a DNA binding domain that recognizes an arbitrary base sequence. When genome editing is planned, TALEN can be activated by dimerization.
Fig.1 A schematic diagram of TALEN. (Malzahn, A., et al., 2017, Cell & bioscience)
The Feature of DNA Binding Domain in TALEN
The DNA binding domain of TALEN is composed of highly conserved repetitive sequences derived from transcriptional activator-like effectors (TALE), in which there is a variable region consisting of two amino acid residues, located at positions 12 and 13, called repeat variable di-residue (RVD) with an amino-terminal domain having a transport signal and a carboxyl-terminal domain having a nuclear localization signal and a transcription activation domain.
The Advantages of TALEN
Easier to Engineer
TALEN is easier to engineer because recognition of each DNA nucleotide requires the use of only one TALE repeat sequence and the corresponding RVD.
More Flexible to Design
TALEN gene editing is more flexible, unlike CRISPR technology which relies on PAM loci, TALEN technology does not rely on specific DNA sequences.
More Efficient in Editing Difficult-To-Edit Genomic Regions
TALENs can be more efficient in editing difficult-to-edit genomic regions such as heterochromatin. Furthermore, TALENs have been shown to exhibit excellent homology-directed repair efficiency because of the closer proximity to the insertion site.
Fewer Off-Target Effects
TALENs exhibit less toxicity in human cell lines because off-target breaks lead to unwanted changes and toxicity in the genome.
Our Services
In principle, the TALEN protein must be redesigned for each target DNA sequence and TALENs can edit arbitrary DNA target sequences. Lifeasible is able to provide efficient and flexible gene editing services based on TALEN technology. We can provide both one-step TALEN services for algae and donor clone design and construction services.
Repetitive Sequences Construction Services
Even though the repetitive sequences of TALE protein make them difficult to construct by polymerase chain reaction (PCR), our company offers expertise in the development of multiple assembly methods based on Golden Gate cloning.
Multiple Gene Editing Services
Our company offers a wide range of TALEN technology-based gene editing services according to homology-directed repair and non-homologous end joining.
- Gene Knock-in
- Create the Premature Stop Codon
- Render Non-Coding RNAs Non-Functional
- Disrupt Control Component
- Delete Pieces of Chromosomes
Why Choose Us
As a well-established and professional CRO company, Lifeasible has the professional equipment and team to support our TALEN services for algae. Please contact us for more information.
Reference
- Malzahn, A., Lowder, L., & Qi, Y. (2017). Plant genome editing with TALEN and CRISPR. Cell & bioscience, 7, 21.
Our services are for research use only and not for any clinical use.