Analysis of Stress-Induced Telomere Depletion

In addition to gradual telomere shortening during cell division, the loss of telomere function can also occur due to stochastic events in which large blocks of telomeric repeat sequences are lost in a single rapid deletion event. In plants, telomeric DNA sequences are essential for chromosome end protection, replication, and cell growth and development regulation. However, under certain stressful conditions, these critical telomeric DNA sequences can be lost, i.e., telomere depletion, leading to genomic instability.

Fig. 1 Telomere loss due to replication problems. (Doksani Y., 2019)

As a leading company in biological research, Lifeasible is equipped with state-of-the-art facilities and a team of highly experienced researchers. We have extensive expertise in studying plant telomeric DNA sequences and their response to stress conditions.

Determination of Telomere Depletion under Stress

Lifeasible utilizes appropriate molecular techniques to investigate the level of telomeric DNA sequence loss under different stress conditions, such as drought, heat, and pathogen infection. One commonly used method is fluorescence in situ hybridization (FISH), which allows the visualization and quantification of telomeric DNA sequences directly on chromosomes. By comparing stressed and non-stressed plant samples, we can assess the extent of telomeric DNA sequence loss and identify any stress-dependent patterns.

Design of Telomere Depletion under Stress

• Based on our many years of experience, we proudly offer our customers experimental protocols to study the deletion of plant telomere DNA sequences under stress. The experimental design is oriented toward exposing plant samples to controlled abiotic or biotic stresses and comparing them to control samples maintained under optimal growth conditions. By measuring telomeric DNA sequences before and after stress induction, the extent of loss can be quantified.
• We aim to ensure the accuracy and reproducibility of our results. Appropriate experimental design includes the selection of stress conditions, appropriate controls, sample collection methods, and data analysis techniques.

Mechanism Analysis of Telomere Depletion under Stress

We help our customers understand the mechanisms underlying stress-induced loss of plant telomeric DNA sequences. One potential mechanism involves telomerase activation, an enzyme responsible for telomere length maintenance. Telomerase activity can be upregulated under stress conditions, leading to increased telomeric DNA synthesis and potential loss due to incomplete replication processes. Additionally, stress-dependent alterations in chromatin structure and epigenetic modifications can also affect telomeric DNA stability.

With our expertise and state-of-the-art equipment, Lifeasible provides valuable insights and solutions for telomere biology. Our researchers stay up-to-date with the latest scientific advancements, ensuring that our analyses and interpretations are based on the most current knowledge in the field. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.