Anthocyanins are water-soluble flavonoid pigments widely found in plants, giving flowers, fruits, and leaves rich colors. They are also of great value in plant stress resistance, antioxidants, and human health. Its biosynthetic pathway involves the metabolism of phenylalanine, which undergoes a multi-step enzymatic reaction to produce a variety of monomer derivatives. Lifeasible has many years of experience in non-targeted metabolomic research and analysis. It is especially suitable for discovering unknown or low-abundance anthocyanin derivatives, which further helps researchers to reveal plant physiological mechanisms, improve quality, and provide key data support.

Explore Our Anthocyanin Non-Targeted Metabolomics Analysis

• Comprehensive coverage. Based on non-targeted metabolomics technology, we qualitatively analyze more than 100 anthocyanin monomers (e.g., Cyanidin, Delphinidin, Pelargonidin, etc.), covering a variety of modifications such as glycosidation and acylation.
• Precise quantification. We incorporate isotopic internal standardization for the relative quantification of key anthocyanin monomers to ensure data accuracy.
• Customized analysis. Provide differential metabolite screening, functional enrichment, and metabolic pathway visualization services according to research needs.
• Sample support. We accept plant tissue (fresh or dried samples are acceptable) and cell and fermentation broth samples.

Application of Anthocyanin Non-Targeted Metabolomics Analysis

• Research on plant growth and development mechanism. Anthocyanins play an essential role in the process of plant growth and development. By analyzing anthocyanin non-target metabolomes, we can gain an in-depth understanding of the changing law of anthocyanin metabolism in the process of plant growth and development and reveal its molecular mechanism in plant growth and development.
• Research on plant resistance to adversity stress. Anthocyanin non-target metabolome analysis can reveal the regulatory mechanism of anthocyanin metabolism in plants under adversity stress and provide a theoretical basis for the cultivation of plant varieties with strong resistance to adversity.
• Plant color metabolism research. Anthocyanin non-target metabolome analysis can be used to study the molecular basis of plant color formation, understand the relationship between anthocyanin metabolism and plant color, and provide a reference for plant breeding and quality improvement.
• Food science research. Anthocyanin non-target metabolome analysis can be used to study the change rule of anthocyanins during food processing and storage, as well as its impact on human health, to provide a scientific basis for the development of functional food.

Sample Request

• Sample type. Plant tissue and liquid samples such as juice, pulp, etc.
• Biological replicates. 6-10 biological replicates are recommended.
• Sample requirement. Plant tissue ≥200mg, liquid type ≥0.5ml.
• Preservation and transportation. Samples can be snap frozen with liquid nitrogen, stored at -80 degrees, or sent directly on dry ice.

Our Service Workflow

• Sample processing. Our professional team performs sample grinding and weighing to ensure the homogeneity and accuracy of the samples, which lays a good foundation for subsequent analysis.
• Metabolite extraction. We extract metabolites, such as anthocyanins, and maximize the preservation of their natural structure and activity to ensure the reliability of subsequent testing.
• Sequencing. The extracted metabolites are accurately sequenced using our state-of-the-art testing equipment, which can simultaneously detect hundreds of anthocyanin-related metabolites.
• Data analysis. Match the metabolite retention time, molecular mass, secondary fragmentation spectra, and other information in our database and provide accurate and comprehensive analysis results.
• Report issuance. According to customer needs, we provide detailed and clear experimental reports, including sample information, experimental methods, data analysis results, conclusions, etc.

Fig.2 Our service process. (Lifeasible)

Highlights of Our Anthocyanin Non-Targeted Metabolomics Analysis

• Professional technical team. With rich experience in metabolomics research, we can provide professional technical consultation and solutions for our clients.
• Advanced technology platform. We are equipped with advanced equipment to ensure the accuracy and reliability of experimental data.
• Rich database resources. We have established a rich local database to provide powerful support for metabolite identification.
• Strict quality control. We adopt strict quality control measures to ensure the stability and reliability of experimental results.

Lifeasible provides reliable technical support as well as advanced solutions for the untargeted metabolomic analysis of anthocyanins. If you are interested, please feel free to contact us.