Cytochrome P450 is a group B cytochrome superfamily protease with heme as a cofactor and is an important heme protein in plant metabolism. Cytochrome P45 has a variety of complex catalytic functions and is involved in the synthesis and metabolism of terpenoids, alkaloids, sterols, fatty acids, and phytohormones in plants. In recent years, the use of bioinformatics tools to study Cytochrome P450 in depth has become a hot research topic.
Lifeasible has an established sequencing laboratory, and data analysis experts focused on providing high-quality cytochrome P450 bioinformatics analysis services. Our customizable analysis services can help clients to elucidate the protein properties, conserved structural domains, and metabolic mechanisms of Cytochrome P450 in plants of interest.
Cytochrome P450 is involved in catalyzing a variety of primary and secondary metabolic reactions in plants. We can perform sequencing services and complete bioinformatics analysis, including but not limited to physicochemical properties, protein signal peptides, protein secondary structure, conserved structural domains, and evolutionary relationships, for various plants, including cabbage, barley, Arabidopsis, and tobacco.
We used the ProtParam online tool to analyze the amino acid sequences obtained and can obtain sequence lengths, amino acid numbers, and pI values for the cytochrome P450 of the sample plants.
We will choose the most suitable sequencing method according to different types of samples, remove the adapter sequence from the obtained data, and splice to get clean data. Then use the ORF finder online tool to predict the ORF of different plant cytochrome P450 nucleic acid sequences.
We usually use Signal P 4.1 Server to analyze the signal peptide in the cytochrome P450 sequence of the plant to be tested. Not all plants have signal peptides, but plants that detect signal peptides can infer the main types of proteins produced, where they are transported, and the reactions they may be involved in.
We will use MEGA 11.0 to construct a phylogenetic tree. Four different algorithms (Maximum Likelihood, Neighbor-joining, Mini-mum Evolution Tree, and Maximum Parsimony) will be used to compare the constructed evolutionary trees and further clarify the evolutionary relationships between plants.
We have complete laboratory facilities and a strict quality management system. We offer optimized protocols and a wide range of bioinformatics tools for different samples to meet all our client's requirements, saving them time and costs.
Lifeasible has been dedicated to providing customizable bioinformatics analysis of plant cytochrome P450 for many years. Please feel free to contact us with your questions, needs, or collaborations.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
