Rapid and Efficient Plant Genetic Transformation Method - RAPID

Plant genetic transformation technology is an essential tool for plant genetic engineering and modern agricultural molecular breeding. However, existing plant genetic transformation protocols are complex and inefficient, which restricts the genetic modification of most resource plants or crops and has become a technical obstacle to the development and utilization of plant resources. Therefore, the development of efficient and multi-application plant genetic transformation technology has become an important research direction in the field of plant or agricultural research.

On January 18, 2024, Plant Communications published a research paper titled "A simple and efficient in planta transformation method based on the active regeneration capacity of plants" online. After years of exploration and research, researchers have successfully established a new plant genetic transformation method (Regenerative Activity-dependent in Planta Injection Delivery, RAPID) based on the plant's active regeneration ability.

This study used sweet potato, a plant with strong regeneration ability, to test multiple delivery methods and found that positive organs and transformed individuals can be quickly obtained through injection delivery of sweet potato stem segments. At the same time, the transformation efficiency was optimized and improved based on Agrobacterium strain, infection concentration, chemical active agents and other method conditions, and the application of a variety of reporter genes and genetic editing tools was completed. Subsequently, genetic and cytological analysis methods were used to confirm the efficient transfection of plant meristems and the rapid regeneration of individual new transformed organs, which are the fundamental reasons for obtaining stable transgenic plants quickly and efficiently. At present, the RAPID method has been successfully applied to vegetatively propagated economic crops or resource plants such as sweet potatoes, potatoes, and vines.

Compared with traditional genetic transformation methods, the RAPID method has higher transformation efficiency (increased by 20 to 100 times), simpler operation procedures (shortened by 2 to 3 times), and does not require expensive and complicated tissue culture processes. The RAPID method overcomes the limitations of traditional methods in rapid genetic transformation and breaks the bottleneck in the development and utilization of various resource plants. Based on the active regeneration ability and asexual reproduction of plants, this method is expected to be applied to a wider range of plant species, and to realize trait improvement and germplasm innovation of more featured resource plants and economic crops, so as to promote the development of basic and applied researches in related fields, and to provide theoretical and methodological support for the efficient genetic improvement of featured plants and the application of modern agricultural breeding.

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Reference:

Mei, G., et al. A simple and efficient in planta transformation method based on the active regeneration capacity of plants. Plant Commun. 2024: 100822.

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