Brassica Oleracea var. Gemmifera Zenker Transformation

Brassica oleracea var. gemmifera zenker, commonly known as brussels sprout, is a biennial herb in the Brassicaceae family. Brussel sprout is mainly consumed for its fresh leaflets formed in the axillary buds of the plant and has the highest protein content of any leafy vegetable. Brussel sprout contains 20 essential vitamins that help boost the immune system and reduce the chance of disease infection. In addition, Brussel sprout is rich in minerals such as Zn, K, Ca, P, Mg, and Mn, which help to promote cell division, repair, and regeneration. Specifically, Brussel sprout contains sulfur ligands. Sulfur ligands are isothiocyanates, natural pesticides in plants and can reduce poisoning, chronic diseases, and gastritis caused by H. pylori. Currently, research on transgenic plants has become a hot area for agricultural improvement. For Brussel sprout, the important improved traits include disease resistance, insect resistance, herbicide resistance, salt tolerance, cold tolerance, and storage tolerance.

What We Offer

Lifeasible has been committed to providing you with one-stop genetic transformation services for brussels sprout, covering experimental design, vector construction, plasmid transformation, positive screening, and transgene identification. We mainly use the mainstream Agrobacterium transformation method to introduce the target gene into the genome of brussels sprout and screen the seedlings that meet the customer's requirements after PCR identification. We offer several Agrobacterium strains (LBA4404, GV3101, EHA105, AGL1, C58, GV2260) and selectable vector markers (Kanamycin, Hygromycin, Phosphinothricin, G418). Our versatile brussels sprout genetic transformation platform can adapt to the various needs of our customers and provides more selectivity. In addition, the various genetic modification services we offer can be viewed by the link below after clicking on it.

Service Flow

1. Sample submission requirements

Customers can provide constructed brussels sprout transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our entire experimental process is standardized and scalable, enabling the customization and improvement of traits in different brussels sprout varieties. Our staff will proactively and regularly keep you informed of the progress and results of your experiments, and you will be notified by email of key points in particular. Our one-stop personalized solutions are designed to simplify your transgenic research to the maximum extent possible, and you will feel relaxed and comfortable as our partner. All experimental procedures will follow strict QC standards and be operated by experienced plant geneticists.

3. Brassica oleracea var. gemmifera zenker transformation results delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• We use the mainstream Agrobacterium infestation method for brussels sprout gene transformation to ensure professional and reliable results.
• We have a wide range of Agrobacterium strains and standardized and custom vectors with various variant selection markers for you to choose from. Alternatively, you can bring in your vectors.
• We offer a one-stop customization service to achieve maximum simplification of your research project and save you valuable time.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Brassica oleracea var. gemmifera zenker transformation. We can provide customized solutions to help you study a wide range of brussels sprout varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.