Brassica Oleracea var. Italic Planch. Transformation

Brassica oleracea var. italic planch., also known as broccoli, is a genus of Brassica in the cruciferous family. The edible part of broccoli is mainly the crunchy flower stems and bulbous flower buds. The nutritional composition of broccoli is complete, including protein, carbohydrates, vitamin A, vitamin C, vitamin B1, vitamin B2, and minerals. In particular, medical studies have confirmed that broccoli contains the anti-cancer substance glucosyl isothiocyanate, which can stop the growth of early cancer cells and has special health benefits. To further improve the nutritional and industrial value of cassava and reduce the cost of the cassava cultivation process, transgenic research on cassava has become a popular field in recent years. Most genetic modifications aim to improve disease resistance, insect resistance, herbicide resistance, salt tolerance, cold tolerance, storage tolerance, and tuber quality of broccoli.

What We Offer

Lifeasible has a mature and stable genetic transformation platform for broccoli that can provide you with efficient and professional genetic transformation services. Our custom genetic transformation experiments are not restricted by variety or season and can be performed at any time of the year. We have comprehensive knowledge and experienced experts in designing and operating genetic transformation experiments on broccoli, mainly based on the Agrobacterium infestation method. First, we use broccoli seedlings as transgenic recipient material. Then, we infiltrated the seedlings with Agrobacterium carrying the target gene vector and inserted T-DNA into the genome of broccoli. Finally, after healing tissue differentiation and screening, we will get positive transgenic cyanosis that meet your needs. In addition, we have the following target gene vector construction services for you to choose from.

Service Flow

1. Sample submission requirements

Customers can provide constructed broccoli transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

We staff our broccoli transgenic services with experienced experts to address your questions and custom needs throughout the process. During the experiment, we will inform our customers at critical points. We also proactively report on the progress of the experiment on a regular basis. The advantage of our one-stop service is that it minimizes your cyanobacterial transgenic research project saving a lot of time and cost. All you need to do is to submit samples and clarify transformation requirements with our experts. Eventually, you just need to wait patiently for positive seedlings and a detailed lab report.

3. Brassica oleracea var. italic planch. transformation results delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• We offer a one-stop service covering all steps of experimental design, vector construction, plasmid transformation, positive graft screening, and characterization of transgenic broccoli.
• We provide services for the transformation of broccoli using multiple genetic engineering techniques to meet different transformation needs.
• Our transformation platform is based on a universal Agrobacterium-mediated transgenic approach and is well suited for the efficient and stable acquisition of superior traits.
• Our genetic transformation projects can be carried out any time of the year, with no restrictions on species or season.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Brassica oleracea var. italic planch. transformation. We can provide customized solutions to help you study a wide range of broccoli varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.