Brassica Rapa Transformation

Brassica rapa, also known as cabbage, is an important green leafy vegetable in the genus Brassica of the cruciferous family. Cabbage has been widely grown around the world. From the nutritional point of view, cabbage is rich in vitamins, dietary fiber, and antioxidants, which can promote intestinal motility and help digestion. In particular, cabbage has a higher vitamin C content than most fruits and is low in calories. In cabbage breeding and production, there is an urgent need for new varieties that are disease-resistant, insect-resistant, resistant to adversity, and have excellent agronomic traits. To further increase the value of cabbage applications and reduce cultivation costs, the currently evolving plant genetic transformation technology offers a new solution.

What We Offer

Lifeasible provides a professional, stable, and efficient cabbage gene transformation system covering the whole process of experiment design, vector construction, plasmid transformation, positive graft screening, and testing. Our cabbage gene transformation technology is based on the Agrobacterium transformation method, in which experts use the U-shaped parts of cabbage cotyledons as transgenic recipient materials. The recipient material is then infiltrated with Agrobacterium carrying the target gene vector to insert T-DNA into the genome. After screening for the corresponding resistance, we obtain independent resistant healing tissues which further differentiate and regenerate into transgenic cabbage plants. You will feel relax and comfortable working with us, with the most professional service and the shortest experimental cycle. We offer a variety of vector construction, as shown below, for you to choose from.

Service Flow

1. Sample submission requirements

Customers can provide constructed cabbage transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)

• Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our cabbage genetic modification service will be staffed with dedicated experts who will provide experimental advice, project consultation, and reports throughout the sales process and progress. We will keep you informed when the experiment reaches a critical point in case you miss it. Our one-stop service exists to ease your research burden and save you time; all you need to do is to submit samples and communicate your needs with us as required. In the end you just need to be patient and you will receive the positive seedlings and a detailed lab report.

3. Brassica rapa transformation results delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed and comprehensive experimental data and reports.

We Do Better

• We are committed to research and development of cabbage regeneration and genetic transformation system for a long time, and our technology is mature and stable, which can guarantee the success rate of the experiment.
• We have perfect service support during the whole experimental process. For example, in the early stage, we provide professional technical consultation and guidance to optimize the project. After the project is completed, we provide the results as agreed and guide the transplantation and survival of transgenic seedlings.
• We used the mainstream Agrobacterium transformation method, which is efficient, stable, based on single-copy insertion and supports multiple resistance screens.
• Our genetic transformation program can be carried out at any time regardless of variety and season.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in brassica rapa transformation. We can provide customized solutions to help you study a wide range of cabbage varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.