Agrobacterium Competent Cells

The genotype of E. coli HT115 is F- mcrA mcrB, IN(rrnD-rrnE)1 rnc14::Tn10(DE3 lavUV5::T7 polymerase). HT115 is an Rnase III-deficient E. coli strain with IPTG-inducible T7 Polymerase activity. It is mainly used for RNAi interference test of C. elegans. The strain could grow normally in LB or 2YT medium, and the presence of Tn10 transposons made it tetracycline resistant. The chromosome of this strain integrated the λ phage DE3 region (DE3 region contains T7 phage RNA polymerase, which can induce a large amount of T7RNA polymerase in the presence of IPTG, thus initiating the expression of ds RNA in nematode), and could express both T7RNA polymerase and Escherichia coli RNA polymerase. It can also be used for protein expression of pET series, pGEX, pMAL and other plasmids.