Insecticidal Proteins

Cry1Ac is a parasporal crystal or insecticidal crystal protein or delta endotoxin produced by Bt, which has insecticidal activity against a variety of pests. Cry1Ac protoxin is activated by the intestinal juice of the target pest to obtain an activated toxin fragment, which binds to the midgut epithelial cells of the pest to form holes, leading to intestinal paralysis and eventually death due to sepsis.

Vip3Aa7 is a non-crystalline protein produced by Bt in vegetative stage and secreted to extracellular, which has insecticidal activity against various pests. The Vip3Aa7 prototoxin is activated by the intestinal juice of the target pest to obtain an activated toxin fragment, which binds to the midgut epithelial cells of the pest to form holes, leading to intestinal paralysis and eventually death due to sepsis.

Cry1Da2 is a parasporal crystal or insecticidal crystal protein or delta endotoxin produced by Bt, which has insecticidal activity against a variety of pests. The Cry1Da2 protoxin is activated by the intestinal juice of the target pest to obtain an activated toxin fragment, which binds to the midgut epithelial cells of the pest to form holes, leading to intestinal paralysis and eventually death due to sepsis.

The herbicide resistance gene (bar gene) is currently the most commonly used herbicide resistance gene and has been successfully used in crops such as wheat, rice, and corn. Cao, Fu and others connected the herbicide resistance gene bxn or bar and the male sterility gene TA29-barnase in series to construct a plant expression vector and introduced it into crops, achieving the maintenance of transgenic male sterile materials in crops. Huang et al. introduced the bar gene into the restorer line of three-line hybrid rice or two-line hybrid rice to produce hybrid rice seeds with herbicide resistance. Recombinant herbicide resistance (Bar) protein is obtained by recombinant expression of the herbicide resistance gene (bar gene) and can be used for herbicide resistance detection, etc. This product is made from a prokaryotic expression system (E.coli) containing a high-efficiency expression of Bar protein gene, which is fermented, separated and highly purified. The molecular weight is 23KDa.

The herbicide resistance gene (pat gene) is currently the most commonly used herbicide resistance gene and has been successfully used in crops such as wheat, rice, and corn. The recombinant herbicide resistance (pat) protein is obtained by recombinant expression of the herbicide resistance gene (pat gene) and can be used for herbicide resistance detection, etc. This product is made from the prokaryotic expression system (E.coli) containing the highly expressed pat protein gene through fermentation, separation and high purification. The molecular weight is 23KDa.