Cationic liposomes are spherical structures containing positively charged lipids. The positive charge of phospholipids allows cationic liposomes to form complexes with negatively charged nucleic acids through ionic interactions. Upon interaction with the nucleic acid, the cationic liposomes form clusters of aggregated vesicles. These interactions allow cationic liposomes to coalesce and encapsulate various in their aqueous compartments or lipid bilayers. These cationic liposome-nucleic acid complexes are also called lipid complexes. Due to the overall positive charge of cationic liposomes interact more readily with negatively charged cell membranes than classical liposomes.
The nature of the lipid formulation depends on the specific composition (cationic, anionic, and neutral lipids). However, the same preparation method is applicable to lipid vesicles of all compositions. The preparation process's general elements include lipids for hydration, stirring for hydration, and reduction of particle size to obtain uniformly distributed vesicles. Cationic liposomes are manufactured in a similar manner to liposomes. Various methods can be used to form cationic liposomes, such as sonication, extrusion, and vortexing. However, the shear forces associated with these methods can destroy the nucleic acid prior to encapsulation.
Lifeasible offers cationic liposome development services based on market and customer needs, combined with the extensive experience of our laboratory staff. Currently, we can develop products such as amino acid peptide modified cationic liposomes; cyclic compound modified cationic liposomes, folic acid modified cationic liposomes, polysaccharide modified cationic liposomes, tartaric acid modified cationic liposomes, and carbamate as linker cationic liposomes. In addition to customization services, we also provide product solutions to solve customers' problems encountered during liposome preparation.
Optimization of cationic liposomes requires reducing the toxicity they bring. The toxicity of cationic liposomes depends on the structure of their hydrophilic head groups; for example, amphiphiles containing quaternary ammonium head groups are more toxic than amphiphiles containing tertiary amines, so when designing cationic liposomes, priority should be given to less toxic groups. We can provide solutions to address cationic liposomal groups' toxicity and help them be better used in various fields.
In addition to the above services, we offer a full range of comprehensive services for cationic liposome morphology, particle size, encapsulation rate, stability, and in vitro release to help you through every step of liposome development.
Lifeasible provides you with services that meet market standards. We perform quality control at every step of the liposome technology development process and rigorous testing of the result to ensure that our stringent quality standards are met. By continuously developing and optimizing our liposome technology, we can guarantee that the service you receive is of the highest quality.
If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.
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