ChIP-Seq of Animal

Chromatin Immunoprecipitation (ChIP), also known as binding site analysis, specifically enriches DNA bound to target proteins in living cells and is a powerful tool for studying protein-DNA interactions in vivo, usually for the exploration of transcription factor binding sites or histone-specific modification sites. Chromatin immunoprecipitation sequencing (ChIP-Seq) technology, which combines ChIP with second-generation sequencing technology, can efficiently detect DNA segments that interact with histones, transcription factors, etc., on a genome-wide scale.

Figure 1. Diagram of the main steps of ChIP-Seq. (Dániel, Bence, et al. 2014)

Lifeasible first specifically enriches the target protein-binding DNA fragments by ChIP and then purifies them and constructs libraries; the enriched DNA fragments are then sequenced in high throughput based on the Illumina system.

Our ChIP-Seq Project for Animals

• Mouse ChIP-Seq
• Rat ChIP-Seq
• Chicken ChIP-Seq
• Pig ChIP-Seq
• Bovine ChIP-Seq
• Sheep ChIP-Seq
• Fish ChIP-Seq
• Marine Invertebrate ChIP-Seq
• Insect ChIP-Seq
• Non-Human Primate ChIP-Seq
• Equine ChIP-Seq

Our ChIP-Seq Service Process

Technical Parameter

• Sequencing platform and method: HiSeq, MGI PE150, SE50
• Sequencing data volume: 5-6 Gb/sample is recommended (about 30-40M Reads)

Applications

• Detection of regulatory elements.
• Detection of transcription factor binding sites.
• Epigenetic mapping at the genome-wide level.
• Mapping of dynamic chromatin during development.
• Testing the effects of drugs or other stimuli on cellular epigenetics.
• Analysis of the mechanisms of epigenetic regulation in diseases or other physiological phenomena, etc.

Advantages of Our High-Throughput ChIP-Seq Service

• Strong analysis capability: We have a professional bioinformatics analysis team provide customized analysis services in addition to standard analysis.
• Cost-effective: We provide comprehensive services at the most favorable price, helping you save your experiment cost-effectively.
• Experienced technology: We have a strong technical team with rich operational experience in nucleic acid sample processing.
• Comprehensive and fast service: We have a perfect biotechnology service system which can provide follow-up service for your subsequent experiments.

Our ChIP-seq projects involve a wide range of reference animal species, such as pigs, mice, rats, zebrafish, and primates. From material selection, and library sequencing, to data analysis, we ensure that each step is scientifically and meticulously designed to guarantee high-quality research results.

Lifeasible uses advanced sequencing platforms to quickly read high-quality sequencing data and ensure efficient data processing and secure data storage. "Scientific protocol design, strict quality control management, professional analysis team, rich project experience, and quality project service" are our ideal. We look forward to cooperating with you; please feel free to contact us.