Coix lacryma-jobi Transformation

Coix lacryma-jobi is a medicinal and food cash crop with important medicinal and very high nutritional value. However, Coix lacryma-jobi has problems such as high stem, easy to fall, and poor disease resistance, which affect its yield improvement and restrict its industrial development. Genetic transformation of Coix lacryma-jobi using advanced biotechnology can solve the problem of improving Coix lacryma-jobi yield and quality.

The agrobacterium-mediated method has the advantages of simple operation, low cost, ability to transform large segments of DNA, genetic stability, low copy number, etc. It has become the most widely used genetic transformation method. Lifeasible using Agrobacterium-mediated genetic transformation method of Coix lacryma-jobi stem tip, using Coix lacryma-jobi stem tip meristematic tissue as the material for genetic transformation can be effectively used to Coix lacryma-jobi research.

What do we offer?

We utilize Agrobacterium-mediated genetic transformation method of Coix lacryma-jobi stem tip to help researchers obtain transgenic plants and provide technical support for the study of genomics and gene function of Coix lacryma-jobi as well as germplasm innovation. In utilizing the Agrobacterium-mediated genetic transformation method of Coix lacryma-jobi, we mainly performed the following experimental procedures.

• Preparation of genetic transformation recipients. After Coix lacryma-jobi seeds sprouted, the stem tip was allowed to grow to 2-3 cm, and the sprouted Coix lacryma-jobi stem tip was transversely cut with a scalpel to expose its apical meristematic tissues for preparation.
• Culture of Agrobacterium. Agrobacterium strains were inoculated into a yep solid medium containing kanamycin and rifampicin and vectors containing thiamphenicol resistance genes to be transferred, and cultured for 2 d. Single colonies were picked into a yep liquid medium containing kanamycin and rifampicin, and the cultured bacterial liquid was added into a new yep liquid medium containing kanamycin and rifampicin and cultured by oscillation after a few hours.
• Genetic transformation of Coix lacryma-jobi. The bacterial solution was poured into the bottle containing the genetic transformation receptor until it was completely submerged, acetosyringone and surfactant were added for vacuum osmosis treatment, and after pouring out the bacterial solution, the seeds were placed on the nutrient soil, then covered with another layer of nutrient soil, and the poured bacterial solution was poured onto the nutrient soil at 28℃, and cultivated in the dark for 3 d.

Why choose us?

• Experienced. We have a professional technical team engaged in plant genetic transformation for many years, which greatly guarantees the success rate of the experiment.
• Perfect service support. We provide professional technical consultation and guidance in the early stage to optimize the project, communicate with each other at any time during the experiment to keep abreast of the progress, and provide the agreed results according to the contract after the completion of the project.
• Efficient experimental process. We can carry out genetic transformation projects at any time, and we use Agrobacterium transformation method, which can carry out Coix lacryma-jobi genetic transformation quickly, efficiently, and stably.

Our service workflow

Lifeasible provides advanced technical support services in Coix lacryma-jobi genetic transformation based on our advanced technology platform and years of experience in research services. Our plant genetic transformation services play an important role in helping researchers understand the growth and development of Coix lacryma-jobi and improve crop quality, as well as helping customers save valuable research time. If you are interested in us, please feel free to contact us.