Construction of High Enzyme-Producing Fungal Strains

Fungi can secrete a large number of enzymes to break down the substances in their environment. The mixture of enzymes secreted by fungi is of great use in many industrial processes. Maximizing the production of secreted enzymes is of great importance. Traditional mutagenesis and screening methods have produced the majority of strains used for the industrial production of enzymes. With the increasing application of DNA recombination technology, protoplast fusion, and histological studies, new methods and strategies for strain improvement are emerging. For those strains with clear biochemical pathways, strain improvement targets can be selected by metabolic engineering strategies, and for strains with unclear biochemical metabolic pathways, genetic selection can also be performed directly by genomic recombination, systems biotechnology, ribosome engineering, and epigenetic modification to obtain desirable mutant phenotypes. Lifeasible is dedicated to making fungal selective breeding more directed and ortho-mutagenic, offering a variety of new methods for constructing highly enzyme-producing fungal strains.

Figure 1. Sugar-sensing proteins in the plasma membrane of fungal cells. (Van Dijck P, et al., 2017)

We use various genetic manipulation techniques to control the expression of multiple genes. These genes are involved in processes that affect the ability of the fungus to sense its environment and regulate the transcription of enzyme-encoding genes and the secretion of proteins. We are committed to using these techniques to rationally engineer improved fungi to provide high fungal nutrient sensing, gene transcription, protein translation, and secretion capabilities.

• Novel mutagenesis breeding. The common means of selection and breeding of high-yielding strains of industrial microorganisms are chemical or physical mutagenesis, genetic engineering breeding, etc. We can provide traditional chemical mutagenesis, UV mutagenesis, radioactive mutagenesis, etc. We can also provide atmospheric and room temperature plasma (ARTP) technology and heavy ion mutagenesis to improve fungal strains. We use ARTP technology and heavy ion mutagenesis to alter the structure and permeability of the cell wall and cytoplasmic membrane, resulting in DNA damage that is more conducive to producing microbial mutants. We have established simple and rapid high-throughput screening methods to help our customers test and screen large numbers of strains.
• Cultivation system optimization. The ideal carbon source, nitrogen source, surfactant, and unknown growth factors can enhance the enzyme production capacity of the strain. At the same time, temperature, pH, and dissolved oxygen levels play important roles in the enzyme production process of fungi. We can help our customers to optimize the fungal culture system and select the appropriate culture conditions for the strain to promote the production and enzyme production of fungal strains within a specific range.
• Genetic engineering. Genetic engineering technology is an efficient and convenient method to improve the enzyme production capacity of fungal strains. We can use recombinant DNA technology, gene and genome editing, and other technologies to perform targeted modifications and modifications of the cellular network of fungal metabolism, gene regulation, and signaling networks. We can improve the ability of the fungus to secrete multiple enzymes by optimizing existing biochemical reactions and metabolic pathways and introducing exogenous metabolic pathways.

Lifeasible is committed to using genetic modification technologies to help customers build high enzyme-producing fungal strains by improving the nutrient sensing, gene translation, protein transcription, and secretion capabilities of fungi. As your trusted partner, we can meet all your fungal phylogenetic analysis needs and provide efficient, high-quality services. If you want to know the details, please contact us.