The CRISPR/Cas9 vector construction service provided by Lifeasible includes gene editing protocol design, target screening and validation, gene editing vector construction, and supporting recombinant vector construction. Our service can provide you with plasmids that can be used for transfection so that you can carry out gene editing experiments directly. You can also choose some of the services according to your needs.
We adopt the pCas9gRNA7 series of vectors, which use the Cas9-HF1 mutant to minimize the off-target rate while ensuring cutting efficiency, significantly improving the success rate of gene editing. Whether you need gene knockout, knock-in, or targeted mutation, the abundant vector types in the pCas9gRNA7 series of vectors can meet your experimental needs.
| Project | Content | Delivery |
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| Gene editing program design |
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| Target screening |
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| Knockout vector building | Selected targets were constructed into the pCas9gRNA7 vector |
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| Recombinant vector building | Matching recombinant vector or knock-in recombinant vector |
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| Recombinant ssDNA template preparation (targeted mutagenesis experiments) | Preparation of transfection grade ssDNA recombinant template | Transfection grade ssDNA 10 μg |
| Name | Applicable |
|---|---|
| pCas9gRNA7 vector | Recombinant knockout/knock-in |
| pCas9gRNA7-GFP vector | Recombinant knockout/knock-in |
| pCas9gRNA7-Puro/Bsd vector | Recombinant point mutation |
| pCas9gRNA7-Puro/Bsd vector | Neighbor-joining knockout |
Recombinant vectors are available with and without fluorescent labels. You can choose according to your needs.
To meet your scientific needs, Lifeasible also offers empty vector services with vector sequences validated by MIT. These vectors contain a 17 bp-1.8 kb expressible linker instead of a customized sgRNA sequence, which you can modify as needed.
| Services | Expression systems | Screening markers |
|---|---|---|
| eSpCas9 and SpCas9 broad plasmid collection |
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| SpCas9 nickase broad plasmid collection |
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| SaCas9 broad plasmid collection |
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Please feel free to contact us with any questions or suggestions regarding CRISPR/Cas9 vectors.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
