CRISPR/Cas9 Vector Building Service

Inquiry

The CRISPR/Cas9 vector construction service provided by Lifeasible includes gene editing protocol design, target screening and validation, gene editing vector construction, and supporting recombinant vector construction. Our service can provide you with plasmids that can be used for transfection so that you can carry out gene editing experiments directly. You can also choose some of the services according to your needs.

CRISPR/Cas9 vector building service

We adopt the pCas9gRNA7 series of vectors, which use the Cas9-HF1 mutant to minimize the off-target rate while ensuring cutting efficiency, significantly improving the success rate of gene editing. Whether you need gene knockout, knock-in, or targeted mutation, the abundant vector types in the pCas9gRNA7 series of vectors can meet your experimental needs.

Project	Content	Delivery
Gene editing program design	Design the program according to the experimental requirements Design 3-5 targets Design matching recombinant vector (optional)	Gene editing experimental program Recommended experimental procedure Positive cell screening and identification methods
Target screening	Synthesize target sgRNA In vitro digestion to verify target cleavage activity	Target cleavage activity report 1-2 highly active targets
Knockout vector building	Selected targets were constructed into the pCas9gRNA7 vector	2-3 μg of target-specific pCas9gRNA7 vector plasmid One strain Transfection grade plasmid 50 μg (optional)
Recombinant vector building	Matching recombinant vector or knock-in recombinant vector	Matching recombinant vector plasmid 2-3 μg One strain Transfection grade plasmid 50 μg (optional)
Recombinant ssDNA template preparation (targeted mutagenesis experiments)	Preparation of transfection grade ssDNA recombinant template	Transfection grade ssDNA 10 μg

Service flow

CRISPR/Cas9 vector building service flow - Lifeasible

Gene editing vector selection

Name	Applicable
pCas9gRNA7 vector	Recombinant knockout/knock-in
pCas9gRNA7-GFP vector	Recombinant knockout/knock-in
pCas9gRNA7-Puro/Bsd vector	Recombinant point mutation
pCas9gRNA7-Puro/Bsd vector	Neighbor-joining knockout

Recombinant vector selection

Recombinant vectors are available with and without fluorescent labels. You can choose according to your needs.

Advantages

The complete suite of services
Specialized target screening service to ensure successful gene editing
Suitable for various gene editing programs
Complete technical support

To meet your scientific needs, Lifeasible also offers empty vector services with vector sequences validated by MIT. These vectors contain a 17 bp-1.8 kb expressible linker instead of a customized sgRNA sequence, which you can modify as needed.

Services	Expression systems	Screening markers
eSpCas9 and SpCas9 broad plasmid collection	Plasmid Lentiviral	Amp, Puro Amp, GFP
SpCas9 nickase broad plasmid collection	Plasmid	Amp Amp, Puro Amp, GFP
SaCas9 broad plasmid collection	AAV	Amp

Please feel free to contact us with any questions or suggestions regarding CRISPR/Cas9 vectors.

For research or industrial raw materials, not for personal medical use!

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