Cucurbita Moschata Transformation

Cucurbita moschata, also known as pumpkin, is a species of the genus Pumpkin in the family Cucurbitaceae. Pumpkins are commonly grown worldwide and have a wide range of applications. The fruit of pumpkin can be used as a food substitute, and various parts of the whole pumpkin plant can also be used for medicinal purposes. Pumpkin seeds contain pumpkin seed amino acids, which have insect-repellent properties. The pumpkin vine has the effect of clearing heat. Pumpkin tips have the effect of calming the fetus. Pumpkin root can cure toothache. With the continuous industrial utilization of pumpkin resources, it has been difficult for the existing pumpkin resources to fully meet the different demands of new variety selection and breeding. Traditional breeding methods have long and difficult cycles, making genetic traits difficult to stabilize. Creating new varieties by means of genetic engineering is an effective way to solve disease problems and an important way to achieve variety improvement.

What We Offer

Lifeasible is committed to providing a one-stop service for pumpkin genetic transformation and cell regeneration, including experimental protocol design, vector construction, PCR identification, positive seedling screening, and post-sales transplant guidance. The standardized pumpkin genetic transformation services are scalable and allow for a variety of personalization. To meet the different needs of different customers, we offer several types of Agrobacterium, including GV3111, LBA4404, and GV3101. In addition, we offer optional marker variants such as Kanamycin, Hygromycin, Phosphinothricin, G418, etc. You can choose the vector construction service you need from the link below. All our experimental steps are operated by a team of knowledgeable and experienced experts, keeping strict QC standards.

Service Flow

1. Sample submission requirements

Customers can provide constructed pumpkin transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)

• Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

The whole process of pumpkin genetic transformation service is equipped with a professional team of experts. In the pre-experimental stage, we provide professional technical consultation and guidance to optimize the project content. In the middle of the experiment, we can communicate with you at any time to keep you informed of the project's progress. After the project is completed, we will provide you with the agreed results on time and guide the transplanting of the transgenic seedlings to ensure their survival. All you need to do is to confirm the final experimental protocol in an in-depth discussion with our experts and submit the pumpkin samples as required.

3. Cucurbita moschata transformation results delivery

• First, you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• Our standardized experimental procedures ensure efficient and reliable genetic transformation of pumpkin, which is the optimal solution based on more than ten years of experience and continuous optimization in genetic transformation services.
• Our pumpkin genetic transformation system is Agrobacterium-mediated, which is a mature and stable technology that can guarantee the success rate of experiments to the greatest extent.
• We can provide genetic transformation experiments for different pumpkin varieties and are not limited to a specific season. Therefore we can carry out the experiments immediately after receiving the order at any time.
• We have a wide range of Agrobacterium strains and standardized and custom vectors with various variant selection markers for you to choose from. Alternatively, you can bring your own vectors.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in cucurbita moschata transformation. We can provide customized solutions to help you study a wide range of pumpkin varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.