Array-based Tests

DNA microarray, also known as DNA chip assay, is a predominant method for the measurement and profiling of gene expressions. As a high-throughput technology, DNA microarray allows detection of the expression of thousands of genes at the same time.

The core component of the DNA microarray is a DNA chip that contains a large number of specific DNA sequences, arranged in arrays that serve as “probes”. A typical microarray experiment requires the following steps (Figure 1):

• Isolation of mRNAs from both experimental and control samples.
• Reverse transcription of purified mRNAs to cDNAs, with fluorescent or radioactive labels added.
• Mixing of labeled cDNA samples with proper hybridization solutions
• Hybridization of sample mixtures to the DNA chip
• Rinsing off unbound cDNAs from the chip
• Automated scan of the hybridized chip for data collection and analysis

  Figure 1. Principle steps for a typical DNA microarray experiment (Kehoe, Villand et al. 1999).

Compared to other gene expression assays, the DNA microarray has the following advantages:

• High-throughput capacity
• Highly reproducible and reliable
• Straightforward and affordable
• Easy data collection and analysis

Lifeasible is a leading plant biotechnology company with rich experiences in microarray-based studies of plant systems. We provide customized DNA microarray services with high quality and efficiency at competitive rates. Moreover, our featured one-stop service covers all steps of the experiment, including probe design, chip fabrication, assay performance, as well as data collection, analysis, and interpretation. With superior expertise in plant genomics and plant molecular biology, our goal is to provide our customers worldwide with diverse research plans and options, capable of serving a wide range of experimental purposes, including but not limited to:

• MicroRNA expression profiling
• Gene expression profiling
• Single-nucleotide polymorphism (SNP) detection
• Comparative genomic hybridization (CGH)
• Genotyping
• Gene ID acquisition
• Chromatin immunoprecipitation (ChIP)-based array
• Alternative splicing detection

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