Customized Services for Mannosylated Exosomes

Exosomes are a kind of vesicle secreted by cells into the extracellular space, with a bilayer membrane structure and teratoid morphology, containing abundant endosomes and involved in the intercellular molecular transfer. Exosomes are widely found in cell culture supernatants or tissue fluids of plants or animals. As a natural endogenous carrier, exosomes are extremely biocompatible. Exosomes have a phospholipid bilayer structure, similar to liposomes, and can be used not only for transporting lipophilic drugs but also for hydrophilic drugs.

Role of Mannosylated Exosomes

Mannosylated exosomes act as carriers for the delivery of drugs to infected cells for therapeutic purposes. In this approach, azide is effectively integrated into the exosome by altering the metabolic processes of the exosome-secreting cell. DBCO-mannosyl ligands are spliced with azide-integrated exosomes via strain-promoted azide-alkyne click chemistry (SPAAC), which confers the advantage of exosome-targeted drug delivery to cells expressing high levels of mannose receptors, as well as the accumulation of drug by cellular transport at the site of bacterial infection to accumulate drugs.

Figure 1. TEM characterization of unmodified and modified exosome formulations. (Yang et al. 2021)

Our Services

Lifeasible offers customization services for mannosylated exosomes, as well as customization of mannose adducts and assays for mannosylated exosomes. We can assist our clients in developing exosomes and promote their therapeutic use as soon as possible. Below is a list of the customization services for exosomes.

• Customization of mannose conjugates

Lifeasible can attach linkers such as DBCO to mannose to facilitate subsequent exosome modification according to customer needs.

• Customization of mannosylated exosomes

Lifeasible offers customization of mannosylated exosomes by incubating DBCO-mannose or other linker-linked mannose with exosomes at room temperature and then dispersing the mannose-adherent exosomes (mannosylated exosomes) in PBS by centrifugation techniques and storing them at low temperature.

• Characterization of Mannosylated Exosomes

Lifeasible offers services for the characterization of mannosylated exosomes, including testing the size distribution, zeta potential, and morphology of exosomes.

In addition to the above services, Lifeasible also provides other services related to exosomes, and we have the expertise to help you solve your project problems. If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.

Service Advantages

• Lifeasible has highly specialized laboratory technicians and operators and a fully equipped and experienced laboratory.
• As an experienced exosome service company, Lifeasible has the expertise and solutions our customer's trust.
• Lifeasible offers a full range of comprehensive services, from customization, purification, identification, optimization, and testing of exosomes.

About Lifeasible

Lifeasible provides quality life science solutions to companies and research institutes, helping life scientists solve problems in the process of research, development, and technology transformation, improving research efficiency, and accelerating the acquisition of research results. In the future, we will continue to uphold the spirit of never-ending and advancing with the times, keep up with the development trend of the life science industry, and provide users with more excellent products and quality services.