Detection of Milk Protein

The total protein content, true protein content, casein content, whey protein content, essential amino acid content, amino acid composition, and enzymatic activity of some protein-based enzymes in milk are essential for milk testing. These indicators can be used to determine the nutritional status, safety, and adulteration of milk and milk products. In addition, some extraneous proteins or extraneous protein-based enzymes are added to milk and milk products to enhance the nutritional value or storability. These added proteins also need to be measured. Lifeasible specializes in milk-related testing and offers services to help determine the protein content, the activity of protein-based enzymes, and the amino acid composition of proteins associated with milk and milk products.

Our Services

We mainly help determine these indicators based on the methods of international standards. We also provide some other convenient methods to determine them.

Determination of milk protein

Protein in milk is an essential nutrient for milk and milk products. We offer several methods to determine the protein in milk and milk products. We mainly base on the international standard Kjeldahl method for the determination of crude protein or true protein. In addition, we also offer methods such as infrared spectroscopy and UV spectrophotometry for determination.

Determination of casein and whey protein

The main proteins in milk are casein and whey protein. We offer three methods to help determine casein and whey protein in milk and milk products, including the Kjeldahl method, the SDS-CGE method, and the HPLC method.

Determination of acid-soluble β-lactoglobulin

Acid-soluble β-lactoglobulin is an essential heat load indicator and a major milk allergen. We help determine its content based on reversed-phase HPLC to evaluate the heat load properties of milk and the content of allergens.

Determination of soy and pea proteins

Due to low prices, soy and pea proteins are often added to milk products. We determine soy and pea proteins mainly based on SDS-CE. We also help detect adulteration of soy and pea proteins by nano-HPLC-MS combined with principal component analysis. The nano-HPLC-MS method can also detect adulteration of other plant proteins.

Determination of hen's egg white lysozyme

Egg white lysozyme is a protein found in egg whites that has antimicrobial properties. It can be used as a natural preservative in milk and milk products. We help determine hen's egg white lysozyme by reversed-phase HPLC with fluorescence detection to determine the presence of egg white and food safety.

Determination of alkaline phosphatase activity

Alkaline phosphatase activity in raw milk can be used to assess the health status of mammals. The determination of its content in heat-treated milk can be used to assess the heat-treatment properties of the milk. We help determine alkaline phosphatase activity based on three methods, including the phenol release method, fluorometric method, and enzymatic photo-activated system method.

Determination of lactoperoxidase activity

We help detect lactoperoxidase activity in milk and milk products based on photometric and high-throughput fluorescence methods. This determination is mainly used to determine the heat-treatment properties of milk.

Determination of the activity of rennets

Chymosin and pepsin are rennets that are important enzymes involved in the cheese-making process. We help determine the total milk-clotting activity of bovine, ovine, and caprine rennets to evaluate the quality of rennets used for cheese making.

Determination of the activity of pregastric lipase

Pregastric lipase activity is also important for cheese making. For evaluating the quality of pregastric lipase preparations, we help determine the activity of pregastric lipase based on the titration of butyric acid produced by the breakdown of triglycerides.

Determination of amino acids

The analysis of amino acids contained in milk proteins contributes to evaluating of nutritional properties of milk and milk products and analyzing protein adulteration. We help determine amino acids mainly based on the UHPLC method. We also offer fluorescence, mass spectrometry, and electrochemical detection to analyze amino acids.

Lifeasible provides comprehensive and professional milk-related protein testing services. We have accumulated a wealth of testing experience, and our skilled test lab technicians are highly trained. Please feel free to contact us for services.