Detection of Plant Nematodes Based on Morphology

Detection and monitoring are the primary measures for preventing and controlling nematode diseases, so the detection and identification of plant nematodes is very important. Morphological detection is a traditional method to observe and analyze morphological characteristics under a microscope. The nematode is identified according to the body length, tail length, tail tip shape, the width of the middle esophageal bulb, location of the vulva, and the presence or absence of the vulva cover.

Lifeasible provides the detection of plant nematodes by light microscopy and scanning electron microscopy to help our customers worldwide in the field of nematode morphology and identification. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Detection of Plant Nematodes by Light Microscopy

• Based on the principle of optics, optical microscopy magnifies tiny objects that cannot be distinguished by the human eye so that people can extract structure information.

Fig.1 The morphology of nematodes under microscope.

• We provide a preliminary morphological structure analysis of the nematodes by light microscopy. The detailed structure of the nematode body can be observed by placing the nematode on the slide and adding 1-2 drops of lactic acid phenol transparent solution.

Detection of Plant Nematodes by Scanning Electron Microscopy

• Scanning electron microscopy is used to observe the external structures, such as the plant nematodes' head, tail, and lateral region.
• We provide the scanning electron microscope observation of nematode morphology. The heads of plant nematodes are cut off, placed directly on a copper platform containing conductive glue, and observe them after air-drying and spraying with gold. The body must be fixed, dehydrated, and dried before gold spraying for observation.

Fig.2 Methods for preparing samples for scanning electron microscopy of plant nematodes.

Statistical Analysis of Morphological Characteristics of Nematodes

• We provide morphological observation and morphometric measurement of female nematodes, mainly observing the head features. The measurement indexes include body length, maximum gross width, length of the mouth needle, height and width of the ball at the base of the mouth needle, DGO value, the distance between the excreta hole and the head end, and others.
• We provide morphological observation and morphometric measurement of male nematodes, mainly observing the head and tail characteristics. The main measurement indexes were body length, maximum gross width, a value, length of the mouth needle, height and width of the base of the mouth needle, DGO value, and length of the syncretic needle.
• We also provide morphological observation and morphometric measurement of the second instar larvae, mainly observing the head and tail characteristics. The main measurement indexes were body length, maximum gross width, a value, mouth needle length, needle base height and width, DGO value, tail length, and tail transparent zone length.

Lifeasible provides cost-effective, high quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.