Detection of Plant Nematodes Based on Protein Analyses

Protein sequence, mass-to-charge ratio, and immunological techniques focus on using unique protein composition and structure to describe nematode species. Due to the redundancy of the genetic code, proteins provide a reduced vocabulary compared to DNA. However, the alphabet is much more complex, utilizing more than 20 characters compared to four DNA bases. In addition, protein structure and post-translational modifications increase the potential diversity that can be used to define nematode species and facilitate identification.

Lifeasible is a leading provider of comprehensive, high-quality services for plant sciences. We provide detection of plant nematodes based on protein analysis for research purposes to customers worldwide. We look forward to working with our customers to discover the secrets of life sciences.

Detection of Plant Nematodes by Two-Dimensional Gel Analyses

• According to the size and mass-to-charge ratio of protein molecules in different nematode species, two-dimensional gel electrophoresis (2-DGE) separation technology can be used to identify them.
• Lifeasible provides two-dimensional gel electrophoresis to assist our customers in the detection of plant nematodes, which has the advantage of being fast, high resolution, and the only technology that can isolate and display thousands of proteins at the same time.

Fig.1 Protein methods with their associated experimental setup.

Detection of Plant Nematodes by Isozyme Analyses

• Enzymatic phenotypes are one of the first non-morphological based methods used for nematode identification. In brief, the technique involves extraction of soluble proteins from whole nematodes in buffer solution, resolution of the resulting extracts by electrophoresis on starch or polyacrylamide gels, followed by staining for specific enzymes.
• We provide detection of plant nematodes based on isozyme analyses for our customers, mainly including esterase, malate dehydrogenase, superoxide dismutase, glutamate-oxaloacetate transaminase, etc.

Detection of Plant Nematodes by Mass Spectral Analyses

• Matrix-assisted laser desorption/ionization (MALDI) is an ionization technique which uses a laser energy-absorbing matrix to generate gaseous ions from large molecules in the solid state.
• The principle of the mass spectrum technique to identify plant nematodes is that specific peaks are generated in protein profiles between different species or populations, which are used as biomarkers to identify different species.
• We provide detection of plant nematodes based on mass spectral analyses to help our customers accurately determine the protein quality of nematode species.

Detection of Plant Nematodes by Serological Analyses

• Immunoassays have great application in agriculture for diagnosing crop diseases, pesticides, and other naturally occurring compounds.
• We provide nematode detection based on serological analyses, including developing poly and monoclonal antibodies.

Lifeasible provides fast turnaround, high-quality services at competitive prices for customers worldwide. Our advanced technical platforms can help our clients solve the problems they may encounter in research based on years of experience. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.