Detection of Plant Nematodes by DNA Microarrays

DNA microarrays are almost made up of molecules that have already been localized or fabricated on glass microscope slides or silicon chips, such as cDNA and nucleotide sequences. It can simultaneously display the expression of thousands of genes at a time. Thus, it is a powerful tool for genetic analysis. It has the capacity to apply many thousands of nucleotides in an ordered array to a surface, thus allowing the parallel interrogation of thousands of different sequences at once. It is currently being applied in the broad areas of biomedical, pharmacological, and genomic research, as well as diagnosis, food safety, and quality.

Lifeasible provides services to customers worldwide covering the detection of plant nematodes by DNA microarrays. We develop an advanced platform equipped with advanced instruments and professional staff for plant science with a high standard. We customize featured services according to the customers' demand.

Detection of Plant Nematodes by DNA Microarrays

• DNA microarray is a collection of pico-moles of microscopic DNA fragments fixed at defined positions on a solid surface such as a glass slide. This method has a high-performance potential and can thus be applied to classify unique and sensitive species.

Fig.1 Representative examples of microarray hybridization results. (François C, et al., 2006)

• Lifeasible provides detection of plant nematodes with DNA microarrays. In brief, DNA, mRNA, or cDNA from plant nematode cells, tissues, and organisms must be labeled and hybridized with microarray DNA using specific fluorescent molecules. The following fluorescent spot images are captured and statistically analyzed in a confocal scanner.

Detection of Plant Nematodes by TaqMan qPCR with DNA Probes

• TaqMan qPCR also uses labeled DNA probes to detect and quantify nematodes. At the beginning of TaqMan qPCR, the labeled probe binds to the template DNA within the primer-defined site. With each PCR cycle, more dye molecules are released, increasing in fluorescence intensity proportional to the number of amplicons synthesized.
• We provide detection of plant nematodes with TaqMan qPCR. This technique is more specific than standard PCR due to the inclusion of probes, and the amount of fluorescence detected can be used to quantify the number of nematodes present in the sample.

Designing and Implementation of DNA Microarrays Experiments

• DNA Probes.
• Application of DNA to the array.
• Target preparation.
• Hybridization.
• Image detection.
• Analysis of results.

Lifeasible offers services covering the functional detection of plant nematodes to meet your research demands. With years of experience in plant science, our professional platforms can help our clients solve various difficulties. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.